Journal: Pharmaceutics

Article Title: Development and Evaluation of a Novel Diammonium Glycyrrhizinate Phytosome for Nasal Vaccination

doi: 10.3390/pharmaceutics14102000

Figure Lengend Snippet: Flow cytometric analysis of CD11c, CD80, and CD86 expression in BMDCs after treatment of blank medium, free DG, and DG-P for 24 h ( n = 4).

Article Snippet: Fluorochrome-labelled anti-mouse monoclonal antibodies including APC-CD11c, FITC-CD80, and PE-CD86, were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Expressing

Journal: Pharmaceutics

Article Title: Development and Evaluation of a Novel Diammonium Glycyrrhizinate Phytosome for Nasal Vaccination

doi: 10.3390/pharmaceutics14102000

Figure Lengend Snippet: Quantification of CD80 and CD86 expression on BMDCs after activation ( n = 4). Note: *, ** and *** represent the differences at p < 0.05, p < 0.01, and p < 0.001, respectively.

Article Snippet: Fluorochrome-labelled anti-mouse monoclonal antibodies including APC-CD11c, FITC-CD80, and PE-CD86, were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Expressing, Activation Assay

Journal: Cell Reports Medicine

Article Title: Macrophages are activated toward phagocytic lymphoma cell clearance by pentose phosphate pathway inhibition

doi: 10.1016/j.xcrm.2024.101830

Figure Lengend Snippet:

Article Snippet: Rat monoclonal anti-CD86 , Miltenyi Biotec , Cat#130-123-724,;RRID: AB_2889634.

Techniques: Staining, Virus, Recombinant, Bicinchoninic Acid Protein Assay, Cell Viability Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Blocking Assay, Phospho-proteomics, Purification, Mass Spectrometry, Software, Modification

Journal: Microorganisms

Article Title: Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation

doi: 10.3390/microorganisms8111776

Figure Lengend Snippet: Differential effects of exosomes secreted by EBV-infected gastric cancer cells on the maturation phenotype of human monocyte-derived dendritic cells (moDCs). Immature DCs were either untreated or treated with the indicated exosomes or with LPS as a positive control for maturation. ( A ) Phase-contrast images of moDCs cultured with exosomes in EBV-infected cells. Large aggregates of cells (arrows) as reported for mature moDCs were found in MKN74-derived exome-treated moDCs but not in EBV-positive cell-derived exosome-treated cells. ( B ) Flow cytometry results of CD1a and CD86 on moDCs incubated with the indicated exosomes. The data are presented as % positive cells of indicated markers. The results are representative of three independent experiments from different donors.

Article Snippet: The primary antibodies used were CD1a-PE (BioLegend, San Diego, CA, USA, clone HI149) and CD86-PE (TONBO Biosciences, San Diego, CA, USA, clone IT2.2).

Techniques: Infection, Derivative Assay, Positive Control, Cell Culture, Flow Cytometry, Incubation

Journal: Microorganisms

Article Title: Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation

doi: 10.3390/microorganisms8111776

Figure Lengend Snippet: Representative figures of dendritic cells expressing each DC marker in gastric cancer tissue. DCs expressing Langerin, CD1a, and S100 were identified as cells with abundant dendrites. DCs expressing BDCA-2, CD83, and CD86 were identified as relatively small round cells (arrowhead). More abundant infiltration of dendritic cells in EBVaGC was observed than in EBV-negative gastric cancer for each DC marker. Immunohistochemical analysis of Langerin, CD1a, S100, CD83, CD86 and BDCA. Scale bar: 50 µm.

Article Snippet: The primary antibodies used were CD1a-PE (BioLegend, San Diego, CA, USA, clone HI149) and CD86-PE (TONBO Biosciences, San Diego, CA, USA, clone IT2.2).

Techniques: Expressing, Marker, Immunohistochemical staining

Journal: Microorganisms

Article Title: Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation

doi: 10.3390/microorganisms8111776

Figure Lengend Snippet: Number of dendritic cells that infiltrated gastric cancer tissue. Dendritic cells expressing each DC marker were counted, and the number per tumor area is shown. The number of DCs is significantly higher in EBVaGC than in EBV-negative gastric cancer for any of the DC markers ( p < 0.001 for Langerin, CD1a, CD83, CD86, and BDCA-2. p < 0.05 for S100, determined by using Wilcoxon rank sum test).

Article Snippet: The primary antibodies used were CD1a-PE (BioLegend, San Diego, CA, USA, clone HI149) and CD86-PE (TONBO Biosciences, San Diego, CA, USA, clone IT2.2).

Techniques: Expressing, Marker

Journal: Microorganisms

Article Title: Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation

doi: 10.3390/microorganisms8111776

Figure Lengend Snippet: Correlation between the number of dendritic cells per tumor area and clinicopathological factors.

Article Snippet: The primary antibodies used were CD1a-PE (BioLegend, San Diego, CA, USA, clone HI149) and CD86-PE (TONBO Biosciences, San Diego, CA, USA, clone IT2.2).

Techniques:

Journal: Microorganisms

Article Title: Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation

doi: 10.3390/microorganisms8111776

Figure Lengend Snippet: Mature DC ratio in DC-high and DC-low cases. CD83/CD1a ratio and CD86/CD1a ratios were significantly lower in CD1a-high cases than in CD1a-low cases ( A , p < 0.01, determined by using Wilcoxon rank sum test). The similar result was also obtained in Langerin ( B , p = 0.08 and p = 0.005, respectively, Wilcoxon rank sum test).

Article Snippet: The primary antibodies used were CD1a-PE (BioLegend, San Diego, CA, USA, clone HI149) and CD86-PE (TONBO Biosciences, San Diego, CA, USA, clone IT2.2).

Techniques: